Fig. 6.

Chronic GLT-induced stress reduces PAK1 abundance concurrent with reduced ERK and Bcl2 phosphorylation. INS 832/13 cells were subjected to GLT for 24 h and (a) cell death was determined by ELISA. (b) Resultant lysates were assessed for PAK1 content, phosphorylated (p) and total (t) forms of ERK and Bcl2, and cleaved caspase 3 by immunoblot quantification. The bar graph represents the average ± SEM, with vehicle-treated lysate bands in each of five independent cell passage experiments set equal to 1 and GLT-treated protein levels normalised thereto. *p < 0.05