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. Author manuscript; available in PMC: 2017 May 10.
Published in final edited form as: Mucosal Immunol. 2016 Oct 19;10(3):716–726. doi: 10.1038/mi.2016.92

Figure 1. IRF5 is a critical component of pulmonary homeostasis and Type 2 responses after HDM exposure.

Figure 1

(A) Experimental design of HDM-induced allergic airways disease. Resistance measured in tracheotomised animals to a dose response (B) or at a representative dose of 30 mg/ml of methacholine (C). Differential cell counts of Wright-Giemsa stained cytospins recovered from the BAL (D) and lung (E). (F) Lung sections stained with H&E; original magnification x40; Scale bar = 50 μm, representative photomicrographs are shown. IL-13 levels in the BAL (G) and Lung eotaxin-2 (H) and as determined by ELISA. (I) CD4+T1/ST2+IL-13+ Th2 cells recovered from the lung and quantified by flow cytometry. Data shown represent means ± standard error mean (s.e.m.), *P < 0.05, **P < 0.01, ****P < 0.0001 WT compared with IRF5-/- animals by Mann-Whitney test. Data were generated from four independent experiments; n=7-20 per group.