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. Author manuscript; available in PMC: 2017 May 10.
Published in final edited form as: Mucosal Immunol. 2016 Oct 19;10(3):716–726. doi: 10.1038/mi.2016.92

Figure 2. IRF5 deficiency leads to augmented mucus and collagen production in the lung after HDM exposure.

Figure 2

Lung sections stained with PAS (A) and Sirius red (B), respectively. PAS staining to identify mucin-containing cells (purple, top panel); Sirius Red staining of lung sections depicts peri-bronchiolar and perivascular collagen (red, bottom panel), original magnification x40; Scale bar = 50 μm, representative photomicrographs are shown. (C) Scoring of lung sections stained with periodic acid-Schiff. (D) Recently synthesized total lung collagen was quantified by a biochemical (Sircol) assay. (E) Quantitative image analysis of sub-epithelial peri-bronchiolar collagen density determined by measuring Sirius red-stained collagen in lung sections under polarized light; data was normalized to WT untreated control and expressed as percent (%) increase. Data shown represent means ± standard error mean (s.e.m.), *P < 0.05, **P < 0.01, WT compared with IRF5-/- animals by Mann-Whitney test. Box and whisker plots represent the mean, IQR and minimum and maximum values. Data were generated from four independent experiments; n=15–20 per group.