(A) Experimental design of HDM-induced allergic airways disease.
Resistance measured in tracheotomised animals to a dose response
(B) or at a representative dose of 30 mg/ml of methacholine
(C); n=4-5 per group. Differential cell counts
of Wright-Giemsa stained cytospins recovered from the BAL (D) and
lung (E); n=4-5 per group. (F) Lung
sections stained with H&E; original magnification x40; Scale bar = 50
μm, representative photomicrographs are shown. (G)
Semi-quantitative scoring of H&E (i) sections. Lung IL-13
(H) and eotaxin-2 (I) levels and as determined by
ELISA. Data shown represent means ± standard error mean (s.e.m.), *P
< 0.05, **P < 0.01, WT compared with IRF5-/- animals by
Mann-Whitney test. Box and whisker plots represent the mean, IQR and minimum and
maximum values. Data were generated from three independent experiments;
n=10-15 per group.