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. Author manuscript; available in PMC: 2017 Jan 25.
Published in final edited form as: Dev Biol. 2016 Nov 29;421(2):219–232. doi: 10.1016/j.ydbio.2016.11.016

Fig. 2.

Fig. 2

Cartilage morphogenetic defects in wls, wnt9a, wnt5b and gpc4 mutants. (A–E) Ventral view of whole-mount 96 hpf Alcian blue stain of wls, wnt9a, wnt5b and gpc4. (F–J) Dissected flat-mounted lateral image of ventral cartilages. (K–O) 40X image of the Meckel’s cartilage lateral view. Length (L) calculated from the midline tip of the Meckel’s cartilage to the middle of the imaginary line (width (W)) between the retroarticular processes. (P) Meckel’s cartilage length/width ratio measured from a clutch of imaged embryos (**p < 0.01, ***p < 0.001, ****p < 0.0001; Kruskal-Wallis test with Dunn’s multiple comparison test. Significance level at p < 0.05). (Q) Number of cells from one-half of Meckel’s cartilage was counted from figures K–O. No statistically significant difference in cell number across wls, wnt9a, wnt5b and gpc4 (p=0.1151, NS; One-way ANOVA). Scale =50 μm..