Table 1.

Summary gene expression comparison within the Meckel’s cartilage.

Markers in visercocranium		wls −/−	wnt9a −/−	wnt5b −/−	gpc4 −/−
Chondrogenesis	sox9a	++	+	+	+
ECM remodeling	collagen type 2a1	+	+	+	+
ECM remodeling	collagen type 10a1a	−	+	−	+
ECM remodeling	collagen type 1a2	−	+	−	+
Osteogenesis	runx2a	++	+	+	++
Joint patterning	bapx1	++	+/−	+	−

Qualitative scoring of whole-mount RNA in situ hybridization expression patterns compared with their WT sibling within a given clutch. Annotation: ‘++’ = high detectable expression, ‘+’ = normal expression, ‘+/− ‘ = low detectable expression and ‘− ‘ = undetectable expression.