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. Author manuscript; available in PMC: 2017 Jul 1.

Published in final edited form as: Hepatology. 2016 Apr 6;64(1):161–174. doi: 10.1002/hep.28522

FIG. 5 — Immunofluorescent imaging of ATP11C and various basolateral bile salt transporters. (A) ATP11C immunostaining is concentrated in the pericentral area of the liver (indicated by GS immunostaining), localizes to basolateral membrane in littermate controls and is absent from ATP11C-deficient livers. Confocal images highlight ATP11C membrane (indicated by a white arrow) and intra-cellular staining (indicated by an asterisk). (B) CDC50A localizes to the basolateral membrane of central hepatocytes and is below detection limits in ATP11C-deficient liver. (C) OATP1B2, OATP1A1, and OATP1A4 immunostaining is detected in the pericentral area of control liver and is hardly detectable in ATP11C-deficient liver. (D) NTCP immunostaining is homogenously detected in control liver and is absent from the pericentral area of the liver lobule in ATP11C-deficient liver (see insert). GS stains are included to indicate the central hepatocytes. Blue: nuclear 4′,6-diamidino-2-phenylindole staining. Bar represents depicted length in μm.

FIG. 5 — Immunofluorescent imaging of ATP11C and various basolateral bile salt transporters. (A) ATP11C immunostaining is concentrated in the pericentral area of the liver (indicated by GS immunostaining), localizes to basolateral membrane in littermate controls and is absent from ATP11C-deficient livers. Confocal images highlight ATP11C membrane (indicated by a white arrow) and intra-cellular staining (indicated by an asterisk). (B) CDC50A localizes to the basolateral membrane of central hepatocytes and is below detection limits in ATP11C-deficient liver. (C) OATP1B2, OATP1A1, and OATP1A4 immunostaining is detected in the pericentral area of control liver and is hardly detectable in ATP11C-deficient liver. (D) NTCP immunostaining is homogenously detected in control liver and is absent from the pericentral area of the liver lobule in ATP11C-deficient liver (see insert). GS stains are included to indicate the central hepatocytes. Blue: nuclear 4′,6-diamidino-2-phenylindole staining. Bar represents depicted length in μm.