Skip to main content
. Author manuscript; available in PMC: 2017 Apr 10.
Published in final edited form as: Nat Mater. 2016 Oct 10;16(2):230–235. doi: 10.1038/nmat4772

Figure 3.

Figure 3

Biochemical and mechanical cues synergistically mediate platelet contraction force. a, Maximal platelet contraction occurs at 75kpa substrate stiffness and 1U/mL thrombin. b, Single platelet force quartiles for physiologically relevant clot stiffnesses and thrombin concentrations. Platelet contractile forces are highest at a substrate stiffness of 75 kPa or at 5U/mL thrombin. A minimum occurs at 25 kPa stiffness and 1U/mL thrombin. c, Exploded view of b to aid visualization. Microenvironmental stiffness has a dominant role over thrombin concentration. Force attenuation at the highest stiffness conditions suggests a mechanically mediated negative feedback mechanism governing the upper limits of platelet contraction. n refers to total number of platelets tested.