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. Author manuscript; available in PMC: 2018 Feb 12.
Published in final edited form as: Biochem Biophys Res Commun. 2016 Oct 13;483(3):923–929. doi: 10.1016/j.bbrc.2016.10.024

Fig. 4. Adenovirus (Ad)-mediated overexpression of GATA-6 not GATA-1 in rabbit colonic SMCs significantly increased constitutive and IL-1β-induced RGS4 promoter activity.

Fig. 4

(A) Fluorescent micrograph showing the efficiency of Ad-EGFP transduction. (B) RT-PCR analysis validated the expression of Ad-GATA-1 and Ad-GATA-6. Ad-GATA-1 inhibited the mRNA expression of endogenous GATA-6. (C, D) RT-qPCR and Western blot analysis for RGS4 mRNA and protein expression. Rabbit colonic SMCs were infected with indicated Ad for 24 h and treated with or without IL-1β (10 ng/ml) for 3 h before total RNA and protein lysates were prepared. * P<0.05 indicates a statistically significant increase by student’s t test compared with Ad-EGFP control. ++ P<0.01 indicates a significant increase in IL-1β treatment group compared with the control group. The number between panels indicates the relative fold of optical density after IL-1β treatment compared with the corresponding control.