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. Author manuscript; available in PMC: 2017 Feb 1.
Published in final edited form as: Innate Immun. 2016 Nov 24;23(2):111–127. doi: 10.1177/1753425916679255

Figure 1.

Figure 1

Avoidance behavior suggests that C. elegans recognizes C. burnetii as a pathogen. Twenty-five wild type C. elegans stage-1 and stage-4 larval populations were separately transferred (in triplicate) to NNGM plates containing four small lawns of different bacterial preparations: (i) live E. coli (CEC); (ii) dead CEC; (iii) live C. burnetii (CCB); and dead CBB. After 24 h at 25°C, the majority of both: (a) L1-stage and (b) L4-stage nematodes were found on live CEC and both life stages appeared to avoid C. burnetii. All experimental groups were compared by one-way ANOVA (P <0.0001 for L4; P =0.0005 for L1), followed by post-test comparison of all groups by Tukey’s test. Both life stages accumulate on live E. coli in significantly greater numbers than on other food sources (P <0.01).