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. Author manuscript; available in PMC: 2017 Feb 1.
Published in final edited form as: Innate Immun. 2016 Nov 24;23(2):111–127. doi: 10.1177/1753425916679255

Figure 8.

Figure 8

Lifespan of C. elegans sek-1(−) is significantly decreased and accompanied by markedly overt intestinal distension and live C. burnetii accumulation. (a) SEK-1 is a MAPK that participates in P. aeruginosa infection of nematodes through an unknown receptor(s) (adapted from Ewbank37). (b) Kaplan–Meier survival plot of wild type or sek-1(−) C. elegans exposed to live E. coli OP50 or CCB. Survival assays comparing sek-1 mutants exposed to live E. coli or CCB showed a significant (P <0.0001) lifespan reduction with median survival times of 7 d on live E. coli and 5 d on live C. burnetii. (c, d) Fluorescence microscopy suggests that premature death of sek-1(−) mutants is associated with pronounced intestinal distension (intestinal lumen outlined with dashed line). Dar pathologies were observed but rare (not shown). SEK-1 nematode imaged after 9 d of CCB exposure. Bar =50 μM. Gr: grinder; An: anus; PC: phase contrast; mCh: fluorescent mCherry signal.