Figure 4.

Twenty-four weeks of high-fat diet decreased cardiac sympathetic tone in male and reduced cardiac vagal tone in female mice. Changes in heart rate in response to (A) mecamylamine [ganglionic blocker (5 mg/Kg i.p)], (B) propranolol [β-adrenergic receptor blocker (6 mg/kg)], and (C) atropine [muscarinic antagonist (5 mg/Kg i.p)] in control- (CD) and high fat-fed (HFD) male and female mice, measured by radio-telemetry in conscious animals. Data are expressed as percentage of basal condition. Data are presented as mean ± SEM. ^*P < 0.05 vs. control diet within sex group; ^†P < 0.05 vs. male/ female within diet group. (n per group: CD male: 5; CD female: 5; HFD male: 6; HFD female: 5)