Figure 4.

Electron micrographs of branchlet internodal cells treated with solvent (A) or ikarugamycin (B–E). (A) Smooth plasma membrane in a cell dark-incubated for 6 days in the presence of 0.05% DMSO (control condition). Thick black arrows indicate remnants of charasomes. (Ch, chloroplast; g, glycosome). (B–D) Cortical cytoplasm in cells dark-incubated for 6 days in the presence of 0.5 μM ikarugamycin (IKA). Large charasomes (thick black arrows) and CPs or CVs (white arrow heads) at the charasomal surface. Typical CPs are present at the smooth plasma membrane (thin black arrow in D) (m, mitochondrion). (E) No effect of IKA (4 μM for 30 min) on the formation of CVs (arrow heads) from the TGN (MVB, multivesicular body). Bars are 500 nm.