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. Author manuscript; available in PMC: 2017 Jan 26.
Published in final edited form as: Stem Cells. 2016 Feb 26;34(6):1615–1625. doi: 10.1002/stem.2305

Figure 2.

Figure 2

Klotho deficiency decreased cell proliferation and pluripotent transcription factor expression in adipose-derived stem cells (ADSCs). (A): MTT assay (optical densities) of the proliferation of ADSCs in WT and KL(−/−) mice over a period of 9 days. (B): Colony formation of ADSCs cultured for 14 days. (C): Western blot analysis of Nanog, Sox-2, and Oct-4 expression. Results were normalized with β-actin. *, p <.05; **, p <.01 versus the WT group. n =3 independent experiments. Abbreviations: KL, WT, wild type.