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. 2016 Dec 3;101(4):1581–1592. doi: 10.1007/s00253-016-8021-7

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© The Author(s) 2016

Open Access This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.

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Fig. 3 — Extracellular alginate depolymerization by AlyB of V. algivorus. a Liquefaction of alginate-containing medium. 1, LB medium with 50 g/l sodium alginate; 2, JM109/pM01 broth with 50 g/l sodium alginate (vector control); 3, JM109/pM02 broth with 50 g/l sodium alginate (whole broth of cells expressing alyB of V. splendidus); 4, JM109/pM03 broth with 50 g/l sodium alginate (whole broth of cells expressing alyB of V. algivorus). b Results of GPC analysis. Black, 2 g/l sodium alginate standard; pink, LB medium; blue, 2 g/l sodium alginate after processing with the supernatant of JM109/pM01; brown, 2 g/l sodium alginate after processing with the supernatant of JM109/pM03