Figure 3.

Subunit Architecture of the FANCB-FANCL-FAAP100 Assembly Studied by XL/MS

(A) Inter-subunit crosslinks between the six-protein BL100 complex. The lengths of the subunits correspond to the lengths of the colored rectangles that they represent: blue, FANCB; green, FAAP100; orange, FANCL. Red lines represent intra-molecular crosslinks. Blue lines represent inter-molecular crosslinks.

(B) Crystal structure of the human FANCL central domain with detected lysine residues highlighted in red (PDB: 3ZQS). All detected crosslinks in the FANCL region covered by the crystal structure map involve lysines that are surface exposed and are spaced within a range compatible with the spacer arm in the BS3 crosslinking agent.

(C) Cartoon representation integrating the crosslinking/mass spectrometry and single-particle analysis characterization of the BL100 architecture. The total number of detected inter-\intra-molecular crosslinks is indicated for each cartoon.

See also Table S1.