Figure 4.

The FANCC-FANCE-FANCF Complex and Its Interaction with FANCB-FANCL-FAAP100

(A) Coomassie-stained SDS-PAGE gel of the CEF complex, containing an MBP N-terminally fused to FANCC.

(B) Negative-stain two-dimensional classes of the of the MBP-CEF complex. Box size is 441 Å × 441 Å.

(C) Coomassie-stained SDS-PAGE gel of the reconstituted and purified MBP-CEF/BL100 assembly.

(D) Negative-stain two-dimensional classes of the reconstituted and purified (MBP)CEF-BL100 complex. Purple arrowheads point at the MBP-CEF density. Due to the flexible nature of the BL100-(MBP)CEF interaction, the MBP-CEF complex appears slightly smaller when BL100 is incorporated. A view of the isolated BL100 is shown, framed in white, for comparison. Box size is 580 Å × 580 Å.

(E) Cartoon representation of the BL100-CEF architecture.

See also Figure S4.