Figure 6.

Three-Dimensional Reconstruction of the CEF-Co-expressed FANCI-FANCD2 Assembly

(A) Representative two-dimensional classes of the CEF-co-expressed ID2 complex matched to the two-dimensional projections of the electron density map generated from the ID2 crystallographic atomic coordinates (PDB: 3S4W). Box size is 350 Å × 350 Å.

(B) Three orientations and a cut-through view of the X. laevis ID2 EM structure with docked mouse ID2 crystal structure. The fit is excellent, in particular, at the dimerization interface. Lysines targeted for mono-ubiquitination are highlighted in red (residue numbering refers to the mouse proteins, and the corresponding lysines in the X. laevis ortholog are indicated in parentheses).

See also Figure S7.