FIG. 8.

Representative confocal images taken on fluorescent dye labeled PC12 cells cultured on NGF-conjugated PPY-PLLA fibers with and without ES: (A) control group with no ES; (B) with ES at 10 mV/cm; (C) with ES at 100 mV/cm. Applied ES changed cell morphology resulting in longer axons (B, C). The effect of neurites extension was inhibited at 1000 mV/cm ES as evidenced though shorter neurites protruding from its cell body (D). These images were analyzed and semiquantitative results are presented as (E) % of neurite bearing cells and average neurite length (F) following ES. These results suggest that PC12 cells stimulated at 100 mV/cm resulted highest number of neurite bearing cells and the largest average neurite length. (Reprinted with permission from Elsevier.)¹⁸