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. 2017 Jan 12;2017:3296498. doi: 10.1155/2017/3296498

Figure 1.

Figure 1

Inflammatory cytokines promote MCP-1 production in SCDC2 cells. SCDC2 cells were stimulated with or without 10 ng/mL of IL-1β, IL-6/sIL-6R, or TNF-α. (a, b) mRNA expression levels were investigated by RT-qPCR using rat-specific primers. Reported values are normalized to Gapdh expression. The results are expressed as the fold change relative to the respective control (-). (c, d) The amount of secreted chemokines was measured using sandwich ELISA kits for rat-specific MCP-1 and SDF-1α. Data are presented as the mean ± standard deviation. ∗P < 0.05 and ∗∗P < 0.01 as compared with the unstimulated control (-).