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Apoptosis-related gene expression. Real-time RT-PCR analysis of the expression of p21, p53, Bcl-2, Bax, and SIRT1 mRNA in ASCs derived from healthy or diabetic donors, cultured in the presence or absence of bFGF for 7 days. Additionally Bcl-2/Bax mRNA expression ratio was calculated, as it negatively correlates with the size of apoptosis. ∗p value <0.05, ∗∗p value <0.01, and ∗∗∗p value <0.001.
