Table 1.
Sequences of primers used in real-time RT-PCR.
| Gene | Sequence 5′-3′ | Amplicon length (bp) | Accession number |
|---|---|---|---|
| p21 | F: AGAAGAGGCTGGTGGCTATTT | 169 | NM_001220777.1 |
| R: CCCGCCATTAGCGCATCAC | |||
| p53 | F: AGATAGCGATGGTCTGGC | 381 | NM_001126118.1 |
| R: TTGGGCAGTGCTCGCTTAGT | |||
| Bcl-2 | F: ATCGCCCTGTGGATGACTGAG | 129 | NM_000633.2 |
| R: CAGCCAGGAGAAATCAAACAGAGG | |||
| Bax | F: ACCAAGAAGCTGAGCGAGTGTC | 365 | XM_011527191.1 |
| R: ACAAAGATGGTCACGGTCTGCC | |||
| SIRT1 | F: ACAGGTTGCGGGAATCCAAA | 155 | NM_001314049.1 |
| R: GTTCATCAGCTGGGCACCTA | |||
| FGFR-1 | F: CCGCCCAACTTTTCCTCCAA | 511 | XM_006716314.1 |
| R: AGGTGGCATAACGGACCTTG | |||
| GLUT-4 | F: CTCTGAGTTGAGGGCAAGGG | 3734 | XM_011523750.1 |
| R: GGGGGACCTACCGCAACATA | |||
| TET2 | F: GAGACGCTGAGGAAATACGG | 258 | NM_001127208.2 |
| R: TGGTGCCATAGGAGTGGACA | |||
| TET3 | F: CAGACCGCTGTGATCGTCAT | 263 | NM_001287491.1 |
| R: AACTTGCGAGGTGTCTTGCT | |||
| GAPDH | F: GTCAGTGGTGGACCTGACCT | 256 | NM_001289746.1 |
| R: CACCACCCTGTTGCTGTAGC |
F: sense primer; R: antisense primer; bp: base pair.