Fig. 2.

791 inhibits HIV-1 replication in PBMCs with limited toxicity. a Average data of HIV-1 BaL virus replication in three different donors over a period of 8 days post-infection (p.i.,) as measured by p24 antigen ELISA (N = 4, 3 donors). PBMCs were infected with HIV-1 BaL (MOI < 0.01) and treated on days 0 and 4 post infection with DMSO, AZT (3.74 μM), 791, 833, or 892 at the concentrations indicated. Error bars indicate standard error of the mean (SEM). The effect of increasing concentrations of 791 (b), 833 (c), or 892 (d) on HIV-1 BaL virion production in PBMCs. Following infection, indicated doses of compound were added, media harvested after 6 days, virus replication measured by p24 antigen ELISA and expressed relative to p24 Gag levels in DMSO-treated cultures (N ≥ 3, *p ≤ 0.05, **p ≤ 0.01, and ***p ≤ 0.001). The effect of the compounds on cell viability was measured by trypan blue exclusion as a percentage of total cells and expressed relative to percent cell viability with DMSO-treatment