Mutants	Primer	Sequence	Position/response element
M1	5′ GGAAATGGTATTGGAGGATCTGGGGCAGCC 3′ 5′ GGCTGCCCCAGATCCTCCAATACCATTTCC 3′	ATTGAGAAATCTGGGGCAG; ATTGGAGGATCTGGGGCAG	−109–(−90); IL-1β/IL-6
M2	5′ GGAAATGGTATCAGGAAATCTGGGGCAGCC 3′ 5′ GGCTGCCCCAGATTTCCTGATACCATTTCC 3′	TGAGAAA; CAGGAAA	−106–(−100); IL-6
M3	5′ GAAAAGCTTAGGGGGTGGTATTGAGAAATC 3′ 5′ GATTTCTCAATACCACCCCCTAAGCTTTTC 3′	GAAATGGT----ATTGAGAA; GAAAAGCT----ATTGAGAA	−116–(−100); IFN-γ
M4	5′ CATTCATGCTGCTAAAGAACATGCCCTCAG 3′ 5′ CTGAGGGCATGTTCTTTAGCAGCATGAATG 3′	TGGGGAA; TAAAGAA	−181–(−175); IL-6
M5	5′ CCCATCTGAAATGCTTCCTCCTACAGGAAG 3′ 5′ CTTCCTGGTAGGAGGAAGCATTTCAGATGGG 3′	GGGGACATTTCA; GG AAGCATTCA	−615–(−604); NF-κB
M6	5′ CTCTAGAAATGAAAGCTTTCCTCAGTGATG 3′ 5′ CATCACTGAGGAAAGCTTTCATTTCTAGAG 3′	GAGGAC-TTTCC; GAAAGC-TTTCC	−750–(−740); NF-κB