Figure 2. Time-dependent HDAC enzyme activity in crude HeLa nuclear lysate at 37˚C±10 μM TSA (HDAC inhibitor). The data represent the formation of deacetylated peptide product quantities by relative fluorescent intensity (RFU) 355 (ex) /460 (em) presented as the mean±S.E.M, n=4. Time-dependent differences in the control were analyzed by a one-way ANOVA, followed by a Tukey post hoc test. *p<0.05. A two-way ANOVA between control and TSA inhibitor established significant differences between the groups *p<0.01.
