Figure 1. Identification and Characterization of ZIKV on RNA Sequencing of Serum from Patient 1.

Panel A shows that 1.5% of complementary DNA sequencing reads were of viral origin (pie chart). Partial external arcs indicate the proportion of viral reads that could be fully classified and human reads derived from messenger RNA transcripts. Viral sequences were identified as Zika virus (ZIKV) by means of Taxonomer software.³ Classification results for viral sequences are shown as a sunburst graph, in which the relative abundance of read-level classification is shown by the size of a given slice, with the highest taxonomic rank in the center and species-level classification in the periphery. The abbreviation ssRNA denotes single-stranded RNA. Panel B shows synonymous and nonsynonymous changes in the ZIKV isolate genome, as compared with its closest taxonomic relatives. Complete sequence information is available at GenBank (accession number, KX827268).