TABLE 2.
Experiment 1, effects of antihypertensive agents enalapril and hydralazine in the drinking water for 16 weeks on the contractility of SHR isolated intact sections of proximal colon ex vivo by KCl; electrical stimulation; and agonists carbachol, angiotensin II, and PGE2 compared with no-treatment SHR control
Results are mean ± S.E.M. from six to eight rats. Statistics were performed using one-way analysis of variance. There were no significant differences between the groups (results not shown).
Control |
Enalapril |
Hydralazine |
|
---|---|---|---|
KCl (40 mM) | |||
Max (mm/g) | 21.2 ± 3.7 | 19.7 ± 3.6 | 20.1 ± 4.5 |
Electrical stimulation | |||
Max (mm/g) | 17.1 ± 1.7 | 14.5 ± 3.4 | 13.5 ± 2.4 |
Carbachol | |||
EC50 (nM) | 159 ± 27 | 186 ± 57 | 207 ± 77 |
Max (mm/g) | 40.7 ± 6.6 | 32.9 ± 5.2 | 41.7 ± 4.4 |
AUC | 79.7 ± 12.8 | 63.5 ± 7.5 | 79.6 ± 11.4 |
Angiotensin II | |||
EC50 (nM) | 2.7 ± 0.5 | 2.7 ± 0.7 | 3.3 ± 0.7 |
Max (mm/g) | 32.7 ± 3.4 | 30.6 ± 4.1 | 32.4 ± 3.0 |
AUC | 72.7 ± 8.7 | 68.2 ± 8.8 | 68.9 ± 7.0 |
PGE2 | |||
EC50 (nM) | 67.3 ± 20.9 | 52.7 ± 17.8 | 208 ± 67 |
Max (mm/g) | 32.2 ± 1.1 | 32.3 ± 2.4 | 32.2 ± 2.8 |
AUC | 74.7 ± 6.5 | 79.1 ± 7.5 | 79.6 ± 11.4 |
AUC, area under the curve as arbitrary units; Max, maximal derived contraction of the tissue.