TABLE 3.
Experiment 2, effects of losartan in drinking water for 16 weeks on SHR contractility of intact ileum and proximal colon sections ex vivo by KCl, agonists (carbachol, angiotensin II, and PGE2), and electrically driven colon and tissue densities compared with no-treatment SHR control
Results are mean ± S.E.M. from six to eight rats. Statistics were performed using Student’s t test.
Control |
Losartan |
P Value |
|
---|---|---|---|
Ileum | |||
KCl (10 mM) Max (mm/g) | 22.6 ± 7.8 | 61.1 ± 10.1 | 0.013 |
KCl (40 mM) Max (mm/g) | 49.3 ± 11.0 | 108.3 ± 12.2 | 0.005 |
Carbachol | |||
EC50 (nM) | 798 ± 343 | 253 ± 23 | ns |
Max (mm/g) | 79 ± 17 | 130 ± 15 | 0.048 |
AUC | 117 ± 33 | 226 ± 29 | 0.033 |
Angiotensin II | |||
EC50 (nM) | 4.7 ± 2.1 | 43.1 ± 13.2 | 0.017 |
Max (mm/g) | 82.5 ± 18.1 | 44.6 ± 11.9 | ns |
AUC | 194.7 ± 55.6 | 49.0 ± 10.5 | 0.028 |
PGE2 | |||
EC50 (nM) | 2383 ± 1081 | 1463 ± 266 | ns |
Max (mm/g) | 48.4 ± 12.3 | 69.1 ± 11.5 | ns |
AUC | 60.1 ± 24.9 | 68.6 ± 11.5 | ns |
Colon | |||
Electrical stimulation | 15.7 ± 5.3 | 14.0 ± 3.0 | ns |
KCl (10 mM) Max (mm/g) | 8.16 ± 3.04 | 4.11 ± 1.16 | ns |
KCl (40 mM) Max (mm/g) | 60.7 ± 9.1 | 57.7 ± 8.1 | ns |
Carbachol | |||
EC50 (nM) | 209 ± 24 | 170 ± 8 | ns |
Max (mm/g) | 74.4 ± 9.0 | 74.1 ± 9.0 | ns |
AUC | 135 ± 16 | 140 ± 18 | ns |
Angiotensin II | |||
EC50 (nM) | 4.66 ± 0.66 | 241.0 ± 91.1 | 0.027 |
Max (mm/g) | 27.1 ± 6.7 | 9.3 ± 2.2 | 0.030 |
AUC | 54.3 ± 14.4 | 4.5 ± 0.9 | 0.006 |
PGE2 | |||
EC50 (nM) | 2078 ± 941 | 934 ± 231 | ns |
Max (mm/g) | 24.9 ± 5.5 | 16.1 ± 2.0 | ns |
AUC, area under the curve (as arbitrary units); Max, maximal derived contraction of the tissue; ns, not significant.