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. 2017 Feb;91(2):145–156. doi: 10.1124/mol.116.106369

Fig. 2.

Fig. 2.

Pronounced differences in early signaling events from the β2-AR. (A) Dose-response curves for a panel of endogenous (Epi, Norepi, Dopa) and synthetic (Iso, Sal, Terb) β2-AR ligands. cAMP accumulation was measured using the luciferase-based biosensor pGLO-20F (Promega), and data were normalized to forskolin-treated controls. Data are average of n = 3–5 experiments ± S.E.M. EC50 curve fitting was performed using Prism6 software. Ligand EC50 (M) and maximum responses (percentage of forskolin response) are summarized under the graph. (B) β2-AR internalization quantified by flow cytometry in cells overexpressing flag-tagged β2-AR. The 1 μM Iso, 1 μM Epi, 10 μM Norepi, 10 μM Terb, 50 nM Sal, and 10 μM Dopa were added for 20 minutes. Data are average from n = 6–23 ± S.E.M. (C) Correlation between cAMP signaling (A) and receptor endocytosis (B).