Fig. 3.

Mass-spectrometric analysis of global phosphoproteomic responses to Iso and Sal. Values from SILAC media swap experiments were log₂-transformed and averaged. (A–C) Scatter plots comparing phosphopeptide abundance for high Iso, low Iso, and Sal. Significantly upregulated sites are shown in red, and downregulated sites are shown in green. Pearson’s correlation values are shown. Dashed lines have a slope of 1 (y = x) for comparison. (D) Average linkage hierarchical clustering was performed using Euclidian distance as a similarity metric on the set of β2-AR–regulated phosphosites. (E) SILAC-LC-MS/MS data and Western blot analysis of phosphoserines in Acly (n = 4–6 ± S.E.M.) and Ctnnb1 (n = 3 ± S.E.M.). Protein levels for the no drug (ND) condition were adjusted to 1, and phosphoprotein changes were normalized to expression levels of GAPDH. Original uncropped Western blots are shows in Supplemental Fig. 4.