Table 1. The sequences of the oligonucleotides used in this study and the combinations of selective primers.
| Type | Name/combination | Sequences |
|---|---|---|
| Adapters | Eco adapter | 5′-CTCGTAGACTGCGTACC and |
| 5′-AATTGGTACGCAGTCTAC | ||
| Hpa/Msp adapter | 5′-GATCATGAGTCCTGCT and | |
| 5′-CGAGCAGGACTCATGA | ||
| Preselective primers | Eco+A | 5′-GACTGCGTACCAATTCA |
| Hpa+A | 5′-ATCATGAGTCCTGCTCGGA | |
| Hpa+C | 5′-ATCATGAGTCCTGCTCGGC | |
| Selective primers | Eco+AC | 5′-GACTGCGTACCAATTCAC |
| Eco+AG | 5′-GACTGCGTACCAATTCAG | |
| Hpa+ATG | 5′-ATCATGAGTCCTGCTCGGATG | |
| Hpa+ATT | 5′-ATCATGAGTCCTGCTCGGATT | |
| Hpa+ACC | 5′-ATCATGAGTCCTGCTCGGACC | |
| Hpa+ACG | 5′-ATCATGAGTCCTGCTCGGACG | |
| Hpa+AAG | 5′-ATCATGAGTCCTGCTCGGAAG | |
| Hpa+CTT | 5′-ATCATGAGTCCTGCTCGGCTT | |
| Hpa+CGC | 5′-ATCATGAGTCCTGCTCGGCGC | |
| Selective primers pairs | Eco+AC and Hpa+ATG; Eco+AG and Hpa+ATG; Eco+AC and Hpa+ATT; Eco+AC and Hpa+ACC; Eco+AC and Hpa+ACG; Eco+AC and Hpa+AAG; Eco+AG and Hpa+CTT; Eco+AC and Hpa+CGC; Eco+AC and Hpa+CTT | |