Fig. 7.

Formation of thicker corneal stromal tissue through stacking (S-SLATEs). SLATEs stacked in groups of five and cultured for 3 weeks were elicited to self-release and recovered as single-block S-SLATEs, imaged by immunofluorescence confocal microscopy following antibody detection of collagen-I and keratocan, and analyzed by tridimensional reconstruction (left panel) and maximum z-stack projection of single-block S-SLATEs (right panel). Scale bar of representative micrograph, 100 μm.