Figure 7.

Single channel currents of wild-type and mutant mucolipin-1 proteins. Representative single channel for wild-type (A) and D1740G mutant mucolipin-1 (B). Tracings were obtained by reconstituting the in vitro-translated protein, in a lipid bilayer exposed to a KCl chemical gradient. Holding potentials are indicated above the tracings. (C) Current-to-voltage relationships for wild-type and D1740G mucolipin-1. Values are the average single-channel currents of seven experiments for the wild-type, and six for the mutant mucolipin-1.