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. 2017 Feb 10;1484:14–25. doi: 10.1016/j.chroma.2016.12.062

Fig. 1.

Fig. 1

IP RP HPLC chromatograms of total RNA extracted from E. coli using Ribopure™ bacterial RNA extraction kit. (a) IP RP HPLC chromatogram of total RNA from E. coli HT115 (DE3) cells transformed with plasmid pCOIV prior to induction. (b) IP RP HPLC chromatogram of total RNA from E. coli HT115 (DE3) cells transformed with plasmid pCOIV following induction with IPTG. The corresponding 5S, 16S and 23S rRNA are highlighted. 2 and 8 μg of total RNA was analysed using gradient condition 1 at 260 nm UV detection.