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. 2017 Feb 10;1484:14–25. doi: 10.1016/j.chroma.2016.12.062

Fig. 2.

Fig. 2

IP RP HPLC chromatograms of total RNA extracted from E. coli using TRIzol® Max™ Bacterial RNA Isolation Kit. (a) IP RP HPLC chromatogram of total RNA from E. coli HT115 (DE3) cells transformed with plasmid pCOIV prior to induction. (b) IP RP HPLC chromatogram of total RNA from E. coli HT115 (DE3) cells transformed with plasmid pCOIV following induction with IPTG. The corresponding rRNA and dsRNA are highlighted. Approximately 15 μg of total RNA was analysed using gradient condition 1 at 260 nm UV detection. (c) IP RP HPLC chromatogram of total RNA extracted from E. coli HT115 (DE3) in conjunction with mechanical shearing and TRIzol. Approximately 10 μg of total RNA was analysed using gradient condition 1 at 260 nm UV detection.