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. 2017 Feb 10;1484:14–25. doi: 10.1016/j.chroma.2016.12.062

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© 2017 The Authors

This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).

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Fig. 4 — IP RP HPLC analyses of dsRNA and ssRNA under denaturing and non-denaturing conditions. (a) IP RP HPLC chromatogram of dsRNA at 50 °C. (b) IP RP HPLC chromatogram of dsRNA at 75 °C. (c) IP RP HPLC chromatogram of ssRNA at 50 °C. (d) IP RP HPLC chromatogram of ssRNA at 75 °C. (e) IP RP HPLC chromatogram of in vitro transcribed dsRNA with an excess of ssRNA. (f) IP RP HPLC chromatogram of purified dsRNA. Following in vitro transcription, the RNA was purified in a single step using RNase T1/DNase in conjunction with solid phase extraction. 2–3 μg of in vitro transcribed ds/ssRNA was analysed using gradient 1 at 260 nm UV detection.