Fig. 5.

RNase A Mass Mapping of the dsRNA using LC ESI MS. (a) Base peak chromatogram of the oligoribonucleotides generated from an RNase A digest of the purified dsRNA. Analysis was performed using LC ESI MS on a maXis ultra-high resolution time of flight instrument. A number of the identified oligoribonucleotides are highlighted. (b) Summary of the RNase mass mapping. Underlined bold = monoisotopic masses correspond to a number of theoretical sequence isomers in either sense or antisense strand. Bold = monoisotopic masses corresponding to a number of theoretical sequence isomers that are unique in either sense or antisense strand. Grey highlight = monoisotopic masses corresponding to single predicted unique oligoribonucleotide sequence in only the sense or antisense strand.