Fig. 6.

RNase T1 Mass Mapping of the dsRNA using LC ESI MS. (a) Base peak chromatogram of the oligoribonucleotides generated from an RNase T1 digest of the purified dsRNA. Analysis was performed using LC ESI MS on a maXis ultra-high resolution time of flight instrument. A number of the identified oligoribonucleotides are highlighted. (b) Summary of the combined RNase A/T1 mass mapping. RNase A fragments are shown in red lines and RNase T1 in blue. Sequences highlighted in green = monoisotopic masses corresponding to a number of theoretical sequence isomers in either sense or antisense strand. Blue = monoisotopic masses corresponding to single predicted unique oligoribonucleotide sequence in only the sense or antisense strand from the RNase T1 digest. Red = monoisotopic masses corresponding to single predicted unique oligoribonucleotide sequence in only the sense or antisense strand from the RNase A digest. (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)