Figure 1.

Generation of _ECP2Y₂R-KO mice. A. The wild type mouse P2Y₂R locus is indicated in the upper panel. The positions of the 5’, 3’ and loxP homology arms used to generating the targeting vector are indicated. Exons 2 and 3 of the gene are indicate by blue boxes. The positions of primers used for genotyping are also indicated. Schematics of the targeted locus, the FLPe deleted locus, in which the neo cassette was removed and the final cre deleted or knockout locus are also shown. Cre-mediated deletion results in excision of the entire coding region of the P2Y₂R gene. B, Immunostaining of P2Y₂R in cross sections of aortic vessels in control, _ECP2Y₂R-KO and P2Y₂R^−/− (total body P2Y₂R knock out) mice. P2Y₂R is expressed in both EC and SMC in aorta of control mice whereas in _ECP2Y₂R-KO mice expression was only detected in SMC. P2Y₂R staining was absent from EC and SMC in the aorta of P2Y₂R^−/−mice. Scale bar represents 50μm. C, Western blot analysis of P2Y₂R expression in primary cultured EC, SMC and PBMC from control and _ECP2Y₂R-KO mice. Data shown are representative of experiments performed from 4 mice for each genotype.