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. 2017 Jan 21;10:483–491. doi: 10.2147/OTT.S127547

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© 2017 Chen et al. This work is published and licensed by Dove Medical Press Limited

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Downregulation of PANDAR in curcumin-treated CRC cells increases apoptosis but attenuates senescence.

Notes: (A) Expression of PANDAR was increased in DLD-1 cells treated with curcumin (5 μM) for 24 h by qRT-PCR. (B) CCK8 assay was used to investigate the proliferation of DLD-1 cells treated with knockdown of PANDAR combined with curcumin. (C) Colony-forming growth assay was used to investigate the proliferation of DLD-1 cells treated with knockdown of PANDAR combined with curcumin. (D) Flow-cytometric analysis was performed to determine the apoptotic percentage of curcumin-treated cells following PANDAR siRNA. (E) SA-β-Gal staining was performed to determine the cellular senescence of curcumin-treated cells following PANDAR siRNA. The experiments were repeated at least three times. *P<0.05, **P<0.01.

Abbreviations: CCK8, Cell Counting Kit-8; CRC, colorectal cancer; DMSO, dimethylsulfoxide; qRT-PCR, quantitative real-time polymerase chain reaction; SA-β-Gal, senescence-associated β-galactosidase; siRNA, small interfering RNA; NS, nonsignificant; FL, fluorescence; OD, optical density; si-NC, small interference-negative control.