Figure 4.

qRT-PCR and Western blotting screening genes associated with the switch from senescence to apoptosis.

Notes: (A) qRT-PCR was used to detect the mRNA levels of FAS, NOXA, PUMA, CCNB1, CDC25C, and CDK1 in the DLD-1 cells treated with negative control, si-PANDAR combined with curcumin, curcumin alone, and si-PANDAR alone. (B) Western blotting analysis showed that the protein levels of PUMA were detected in DLD-1 negative control, si-PANDAR combined with curcumin, curcumin alone, and si-PANDAR cells. The experiments were repeated at least three times. *P<0.05, **P<0.01.

Abbreviations: qRT-PCR, quantitative real-time polymerase chain reaction; si-NC, small interference-negative control.