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. 2017 Jan 26;12(1):e0170709. doi: 10.1371/journal.pone.0170709

Fig 3. Pull-down assay using biotinylated BENDA.

Fig 3

(A) Chemical structure of Bio-BENDA. (B) Pull-down assay using Bio-BENDA. MDA-MB-468 cells were treated with Bio-BENDA in the presence or absence of excess free BENDA for 4 h. Cellular STAT3 was immunoprecipitated with anti-STAT3 or anti-STAT1 antibody and Bio-BENDA binding to STAT3 was detected with streptavidin-HRP. (C) Pull-down assay using Bio-BENDA. MDA-MB-468 cells were treated with Bio-BENDA in the presence or absence of excess free BENDA or HP2 for 4 h. Cellular STAT3 was immunoprecipitated with anti-STAT3 antibody and Bio-BENDA binding to STAT3 was detected with streptavidin-HRP.