Skip to main content
. 2017 Jan 26;12(1):e0170709. doi: 10.1371/journal.pone.0170709

Fig 5. Inhibition of STAT3 function in cancer cells by BENDA.

Fig 5

(A) Effect of BENDA on c-myc promoter binding by STAT3. MDA-MB-468 cells were treated with BENDA or HP2 for 24 h. The ChIP assay was performed using an ExactaChIP Human/Mouse STAT3 ChIP kit. IgG was used as a negative control. (B) Effect of BENDA on c-myc mRNA expression. MDA-MB-468 cells were treated with the indicated concentrations of BENDA or HP2 for 8 h. The mRNA expression levels of c-myc were evaluated by RT-PCR. The means ± SD of three separate experiments are plotted (*P < 0.05 and **P < 0.01 versus vehicle; Student’s t-test). (C) Effect of BENDA on Tyr705 phosphorylation of STAT3 and the expressions of STAT3 and c-Myc protein. MDA-MB-468 cells were treated with the indicated concentrations of BENDA or HP2 for 24 h and lysed for western blotting analysis.