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. 2017 Jan 26;12(1):e0170924. doi: 10.1371/journal.pone.0170924

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© 2017 Wang et al

This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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Fig 5 — B16-F0 cells were scratched and treated with 0.1% DMSO (negative control) or IC₅₀ values of cisplatin (10 μM) or EE-AC (50 μg/mL). Inhibition of migration was observed using a phase contrast microscope (100 × magnification) at 0, 6, 12 and 18 h (Top panel), and the closure of the wound area was calculated (bottom panel). Values are significantly different by comparison with the cisplatin group. *p < 0.05; ***p < 0.001.