Table 2. Effects of cisplatin and the ethanolic extract of Antrodia camphorata fruiting body (EE-AC) on cell cycle regulation in B16-F0 cells.
| Phase | Treatment | ||
|---|---|---|---|
| Control (0.1% DMSO) | Cisplatin (10 μM) | EE-AC (50 μg/mL) | |
| Sub-G1 | 0.06 ± 0.01% | 1.13± 0.09%* | 4.03 ± 0.23%** |
| G0/G1 | 57.6 ± 1.74% | 58.2 ± 2.55% | 49.4% ± 3.19% |
| S | 31.2 ± 1.53% | 31.5 ± 2.02% | 34.5 ± 1.97% |
| G2/M | 10.2 ± 0.37% | 9.3 ± 0.26% | 12.1 ± 0.88% |
Cell cycle analysis of B16-F0 cells after treatment with DMSO (0.1%), cisplatin and EE-AC (IC50) for 48 h. After drug treatment, cells were examined with PI fluorescence and analyzed by flow cytometry. The data were normalized and calculated as a percentage of the control value and they represent the mean ± SD.
Significantly different from the control group at
*p<0.05,
**p<0.01.