Fig 1. pPICZαA expression vector according to the EasySelect Pichia Expression Kit Manual (Invitrogen) and a schematic representation of TEG4-2c scFv with the protein sequence.

(A): All the featured restriction sites are unique. 5´ AOX1: promoter region of AOX1; TT AOX1: transcription termination of AOX1; PTEF1: promoter of TEF1; PEM7: promoter of EM7; Zeocin resistance: Sh ble ORF; CYC1 TT: transcription termination of CYC1. (B): The TEG4-2c scFv coding sequence was cloned between PmlI and XbaI sites and the protein sequence of recombinant tag-scFv including the 6His-tag and the 2 cysteine are highlighted in green. The α-factor signal sequence is represented in blue and the C-myc tag is highlighted in yellow.