Fig 5. Virus titers of HSV/AAV hybrid vectors harboring the mutant rep68/78-D371Y gene are significantly higher than titers of hybrid vectors harboring the wt rep68/78 gene.

(A) Packaging and titration of the HSV/AAV hybrid vectors pHyRaNGFPa and pHyRD371YaNGFPa was performed as described for the HSV-1 vector pHSVGFP (Fig 2C). The data are shown as means ± SE from three independent experiments (*, P<0.05). (B) Western analysis to confirm Rep expression in HSV/AAV hybrid vector producing Vero-2-2 cells. Production of vectors was performed as described for Fig 2C and Fig 5A. Instead of harvesting vector particles, the cells were processed for and subjected to Western analysis. In addition to the Rep-specific antibody to detect Rep expression, the blots were stripped and re-stained with an antibody specific for GFP as a loading and expression control.