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. 2017 Jan 26;12(1):e0170897. doi: 10.1371/journal.pone.0170897

Fig 4. Cross-reactivity of allele-specific TaqMan probes for KRAS mutation subtypes.

Fig 4

(A) DNA extracted from HPAF-II (G12D KRAS mutation; heterozygous with 3:1 mutant allele specific amplification), CFPAC (G12V KRAS mutation: heterozygous with 1:1 mutant to WT alleles), MDAMB-231 (G13D KRAS mutation) and Jurkat cells (WT KRAS) were analyzed by dPCR using the KRAS_520 probe that is specific for G12V mutant subtype. Presence of the FAM fluorescence signal, which is indicative of mutant KRAS allele, was seen only in the dPCR reaction that used DNA extracted from CFPAC cells. (B) The same groups of DNA were analyzed using the KRAS_521 probe that is specific for G12D mutation subtype. As expected, FAM signal was seen only in the dPCR reaction that used DNA extracted from HPAF-II cells.