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. 2017 Jan 26;12(1):e0170897. doi: 10.1371/journal.pone.0170897

Table 1. dPCR KRAS mutation analysis in FFPE EUS-FNA pancreas biopsy specimens.

Cytological Diagnosis
Adenocarcinoma (N = 19) Atypical cells (N = 10) Negative for malignancy (N = 5)
Age (years, SD) 69 (10.8) 71 (10.4) 64 (15.6)
Surgically resected (N, %) 20 (100%) 10 (100%) 0
Surgical pathology diagnosis (N) PDAC (19)
  • PDAC (1)

  • MD-IPMN gastric subtype (5)

  • BD-IPMN gastric subtype (3)

  • PanIN (1)

N/A (no surgery)
FFPE EUS-FNA block cellularity (N, %) [number of cells/10-um section]
100–200 7 (31%) 2 (22%) 0
201–400 5 (23%) 2 (22%) 2 (40%)
401–600 5 (23%) 3 (33%) 1 (20%)
>600 5 (23%) 2 (22%) 2 (40%)
dPCR KRAS mutation analysis (N)
Mutated KRAS 19 9 0
WT KRASa 0 1b 5

adPCR indicates absence of G12D or G12V mutation

b WT KRAS found in the patient with BD-IPMN

Abbreviations: dPCR; digital PCR, WT; wild-type, PDAC; pancreas adenocarcinoma, MD-IPM; main duct intraductal papillary mucinous neoplasm, BD-IPMN; branch duct intraductal papillary mucinous neoplasm, PanIN; pancreas intraepithelial neoplasia