Table 2. Comparison of KRAS mutation analysis in FFPE EUS-FNA by dPCR and surgically resected tissue by Sanger sequencinga.
dPCR in EUS-FNA FFPE sections | ||||
---|---|---|---|---|
KRAS mutation status | WT | G12V or G12Db (1 mutation) | G12V and G12D (2 mutations) | |
Sanger sequencing in surgically resected tissues | WT | 1 | 0 | 0 |
G12V or G12D (1 mutation) | 0 | 22 | 4 | |
G12V and G12D (2 mutations) | 0 | 0 | 1 |
aCytologically benign samples are excluded from this table as none of these patients underwent surgical resection
bKRAS mutation subtypes matched between dPCR and Sanger sequencing for all patients in this category
Abbreviations: dPCR; digital PCR, WT; wild-type